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Histochemical staining of clonal mammalian cell lines expressingE. coli β galactosidase indicates heterogeneous expression of the bacterial gene

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Somatic Cell and Molecular Genetics

Abstract

An evaluation has been made of the E. coliβ -galactosidase (β-gal) gene for use as a reporter gene in mammalian cells in culture. We have adopted a histochemical procedure which enables identification of those cells within a population that express the introduced bacterial gene. Data is presented concerning the sensitivity of the histochemical method relative to an immunological method of detection. It has been found that several clonal cell lines generated after transfection of human 293 cells with a Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter-β-gal construction are mosaic for expression of the introduced mini-gene. Furthermore, after treatment of these clonal cell lines with the nucleoside analog 5-aza-cytidine (5-aza-C), an increase in production of β-gal under control of this promoter element was observed.

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Authors and Affiliations

  1. Institute for Molecular Genetics and Howard Hughes Medical Institute, Baylor College of Medicine, One Baylor Plaza, 77030, Houston, Texas

    Grant R. MacGregor & C. Thomas Caskey

  2. Department of Biochemistry, School of Biology, University of Sussex, BN1 9QG, Falmer, Brighton, England

    Anne E. Mogg & Julian F. Burke

Authors
  1. Grant R. MacGregor
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  2. Anne E. Mogg
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  3. Julian F. Burke
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  4. C. Thomas Caskey
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MacGregor, G.R., Mogg, A.E., Burke, J.F. et al. Histochemical staining of clonal mammalian cell lines expressingE. coli β galactosidase indicates heterogeneous expression of the bacterial gene. Somat Cell Mol Genet 13, 253–265 (1987). https://doi.org/10.1007/BF01535207

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  • DOI: https://doi.org/10.1007/BF01535207

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