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Cholesterol absorption and sterol balance in normal subjects receiving dietary fiber or ursodeoxycholic acid

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Abstract

Twelve normal subjects were placed on a constant diet and evaluated during three treatment periods, each lasting four weeks. Patients received regular diet alone during period A, regular diet plus 60 g/day wheat bran during period B, and regular diet plus 15 mg/kg/day ursodeoxycholic acid during period C. Cholesterol absorption, plasma lipid concentration, daily fecal excretion of neutral and acidic sterols, and sterol balance were determined following each treatment. No changes in serum lipid levels are observed in the three study periods. Bran administration increases fecal neutral sterol excretion and decreases cholesterol absorption (from 50.1 to 42.0%) with respect to period C. Ursodeoxycholic acid administration has no effect on fecal neutral sterol excretion, whereas bile acid excretion is markedly increased. Sterol balance (evaluated as neutral and acidic fecal sterols excreted per day minus dietary cholesterol plus ursodeoxycholic acid given per day) is lower during ursodeoxycholic acid feeding (360±145 mg/day) than during bran feeding (593±174 mg/day). In conclusion, ursodeoxycholic acid decreases sterol balance values; bran-rich diet increases sterol balance and reduces cholesterol absorption.

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References

  1. Siperstein MD, Chaikoff JL, Reinhardt WO: C14-cholesterol. V. Obligatory function of bile in intestinal absorption of cholesterol. J Biol Chem 198:111–114, 1952

    PubMed  Google Scholar 

  2. Westergaard H, Diestschy JM: The mechanism whereby bile acid micelles increase the rate of fatty acid and cholesterol uptake into the intestinal mucosal cell. J Clin Invest 58:97–108, 1976

    PubMed  Google Scholar 

  3. Westergaard H: Duodenal bile acid concentrations in fat malabsorption syndromes. Scand J Gastroenterol 12:115–122, 1977

    PubMed  Google Scholar 

  4. Lutton C, Mathé D, Chevallier F: Vitesse des processus de renouvellement du cholesterol contenu dans son espace de transfer chez le rat. VI. Influence de la ligature du cholédoque et de l'ingestion d'acides biliares ou de cholestryamine. Biochim Biophys Acta 306:483–496, 1973

    PubMed  Google Scholar 

  5. Ponz de Leon M, Carulli N, Loria P, et al: The effect of chenodeoxycholic acid (CDCA) on cholesterol absorption. Gastroenterology 77:223–230, 1979

    PubMed  Google Scholar 

  6. Ponz de Leon M, Carulli N, Loria P, et al: Cholesterol absorption during bile acid feeding. Effect of ursodeoxycholic acid (UDCA) administration. Gastroenterology 78:214–219, 1980

    PubMed  Google Scholar 

  7. Tangedahl TN, Thistle JL, Hofmann AF, et al: Effect of β-sitosterol alone or in combination with chenic acid on cholesterol saturation of bile and cholesterol absorption in gallstone patients. Gastroenterology 76:1341–1346, 1979

    PubMed  Google Scholar 

  8. Einarsson K, Grundy SM: Effects of feeding cholic and chenodeoxycholic acid on cholesterol absorption and hepatic secretion of biliary lipids in man. J Lipid Res 21:23–34, 1980

    PubMed  Google Scholar 

  9. Sama C, LaRusso N: Effect of deoxycholic, chenodeoxycholic, and cholic acids on intestinal absorption in humans. Mayo Clin Proc 57:44–50, 1982

    PubMed  Google Scholar 

  10. LaRusso NF, Thistle JL: Effect of litholithic bile acids on cholesterol absorption in gallstone patients. Gastroenterology 84:265–271, 1983

    PubMed  Google Scholar 

  11. Persson I, Raby K, Fonns-Bech P, et al: Bran and blood lipids. Lancet 2:1208–1208, 1975

    Google Scholar 

  12. Truswell AS, Kay RM: Bran and blood lipids. Lancet 1:367–367, 1976

    Google Scholar 

  13. Kay RM, Truswell AS: Effect of wheat bran fibre on gastrointestinal function, plasma lipids and steroid excretion in man. Br J Nutr 37:227–234, 1977

    PubMed  Google Scholar 

  14. Southgate DAT, Branch WJ, Hill MJ, et al: Metabolic response to dietary supplements of bran. Metabolism 25:1129–1135, 1976

    PubMed  Google Scholar 

  15. Eastwood M, Mowbray L: The binding of the components of mixed micelle to dietary fiber. Am J Clin Nutr 29:1461–1467, 1976

    PubMed  Google Scholar 

  16. Vahouny GV, Roy T, Gallo LL, et al: Dietary fibers. III. Effects of chronic intake on cholesterol absorption and metabolism in the rat. Am J Clin Nutr 33:2182–2191, 1980

    PubMed  Google Scholar 

  17. Vahouny GV, Tombes R, Cassidy MM, et al: Dietary fibers. IV. Binding of fatty acids and monolein from mixed micelles containing bile salts and lecithin. Proc Soc Exp Biol Med 166:12–16, 1981

    PubMed  Google Scholar 

  18. Salvioli G, Salati R, Pastorello M, et al: Cholesterol, bile acid and bile salt adsorption to branin vitro. Pharm Res Commun 13:413–421, 1981

    Google Scholar 

  19. Grundy SM, Ahrens EH, Jr: Measurements of cholesterol turnover, synthesis, and absorption in man, carried out by isotope kinetic and sterol balance methods. J Lipid Res 10:91–107, 1969

    PubMed  Google Scholar 

  20. Rouser G, Kritchevsky G, Yamamoto A: Lipid Chromatographic Analysis, Vol I. New York, Dekker, 1967, pp 99–162

    Google Scholar 

  21. Zilversmit DB: A single blood sample dual isotope method for the measurement of cholesterol absorption in rats. Proc Soc Exp Biol Med 140:862–865, 1972

    PubMed  Google Scholar 

  22. Samuel P, Crouse JR, Ahrens EH Jr: Evaluation of an isotope ratio method for measurement of cholesterol absorption in man. J Lipid Res 19:82–93, 1978

    PubMed  Google Scholar 

  23. Miettinen TA, Ahrens EH Jr, Grundy SM: Quantitative isolation and gas-liquid chromatographic analysis of total dietary and fecal neutral steroids. J Lipid Res 6:411–424, 1965

    Google Scholar 

  24. Grundy SM, Ahrens EH, Jr, Miettinen TA: Quantitative isolation and gas-liquid chromatographic analysis of total fecal bile acids. J Lipid Res 6:397–410, 1965

    Google Scholar 

  25. Arca M, Montali A, Ciocca S, et al: An improved gas-liquid chromatographic method for the study of fecal neutral sterols. J Lipid Res 24:332–335, 1983

    PubMed  Google Scholar 

  26. Salvioli G, Salati R: Faecal bile acid loss and bile acid pool size during short-term treatment with ursodeoxycholic and chenodeoxycholic acid in patients with radiolucent gallstones. Gut 20:698–704, 1979

    PubMed  Google Scholar 

  27. Bolin DW, King RP, Klosterman EW: A simplified method for the determination of chromic oxide (Cr2O3) when used as an index substance. Science 116:634–635, 1952

    PubMed  Google Scholar 

  28. Kirk RE: Experimental Design: Procedure for the Behavioral Sciences Brooks (ed). Belmond, California, Cole Publishing, 1968, p 90

    Google Scholar 

  29. Simons LA, Gayst S, Balasubramaniam S, et al: Long-term treatment of hypercholesterolemia with a new palatable formulation of guar gum. Atherosclerosis 45:101–108, 1982

    PubMed  Google Scholar 

  30. Samuel P, McNamara DJ, Ahrens EH Jr, et al: Further validation of the plasma isotope ratio method for measurement of cholesterol absorption in man. J Lipid Res 23:480–489, 1982

    PubMed  Google Scholar 

  31. Leiss O, Von Bergmann K: Different effects of chenodeoxycholic acid and ursodeoxycholic acid on serum lipoprotein concentrations in patients with radiolucent gallstones. Scand J Gastroenterol 17:587–592, 1982

    PubMed  Google Scholar 

  32. Schoenfield LJ, Lachin JM: Chenodiol (chenodeoxycholic acid) for dissolution of gallstones: the National Cooperative Gallstone Study. A controlled trial of efficacy and safety. Ann Intern Med 95:257–282, 1981

    PubMed  Google Scholar 

  33. LaRusso NF, Srczepanik PA, Hofmann AF: Effect of deoxycholic acid ingestion on biliary acid metabolism and biliary lipid secretion in normal subjects. Gastroenterology 72:132–140, 1977

    PubMed  Google Scholar 

  34. Carulli N, Ponz de Leon M, Zironi F, et al: Bile acid feeding and hepatic sterol metabolism: Effect of deoxycholic acid. Gastroenterology 79:637–641, 1980

    PubMed  Google Scholar 

  35. Isaksson G, Lundquist I, Ihse I: Effect of dietary fiber on pancreatic enzyme activityin vitro. The importance of viscosity, pH, ionic strength, adsorption, and time incubation. Gastroenterology 82:918–924, 1982

    PubMed  Google Scholar 

  36. Mok HYI, Von Bergmann K, Grundy SM: Effect of continuous and intermittent feeding on biliary lipid output in man: Application for measurements of intestinal absorption of cholesterol and bile acids. J Lipid Res 20:389–398, 1979

    PubMed  Google Scholar 

  37. Duane WC, Bond JH: Prolongation of intestinal transit time and expansion of bile acid pool by propantheline bromide. Gastroenterology 78:226–230, 1980

    PubMed  Google Scholar 

  38. Raymond TL, Connor WE, Lin DS, et al: The interaction of dietary fibers and cholesterol upon the plasma lipids and lipoproteins, sterol balance, and bowel function in human subjects. J Clin Invest 60:1429–1437, 1977

    PubMed  Google Scholar 

  39. Tarpila S, Miettinen TA, Metasaranta L: Effect of bran on serum cholesterol, faecal mass, fat, bile acids and neutral steroids and biliary lipids in patients with diverticular disease of the colon. Gut 19:137–145, 1978

    PubMed  Google Scholar 

  40. Avgerinos GC, Fuchs HM, Floch MH: Increased cholesterol and bile acid excretion during a high-fiber diet. Gastroenterology 72:1026, 1972 (abstract)

    Google Scholar 

  41. Walters RL, McLean-Baird I, Davies PS, et al: Effect of two types of dietary fiber on faecal steroid and lipid excretion. Br Med J 2:536–538, 1975

    PubMed  Google Scholar 

  42. Von Bergmann K, Gutsfeld M, Schulze-Hagen K, et al: Effect of ursodeoxycholic acid on biliary lipid secretion in patients with radiolucent gallstones.In Biological Effects of Bile Acids. G Paumgartner, A Stiehl, W Gerok (eds). Lancaster, UK, MTP Press, 1979, pp 61–66

    Google Scholar 

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Salvioli, G., Lugli, R. & Pradelli, J.M. Cholesterol absorption and sterol balance in normal subjects receiving dietary fiber or ursodeoxycholic acid. Digest Dis Sci 30, 301–307 (1985). https://doi.org/10.1007/BF01403837

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  • DOI: https://doi.org/10.1007/BF01403837

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