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Correlation betweenin vitro andin vivo drug metabolism rate: Oxidation of ethoxybenzamide in rat

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Abstract

In vitroand in vivocorrelations of the microsomal oxidation of drugs were examined, using ethoxybenzamide as a model drug. Ethoxybenzamide disappearance time course from rat plasma in vivowas analyzed by a two-compartment model assuming a Michaelis-Menten type elimination process. Ethoxybenazmide oxidation in vitrowas measured by the appearance rate of salicylamide in rat liver microsomal suspension. Parameters obtained were Vmax=3.46 and 3.77 μmoles/min/kg body weight and Km=0.378 and 0.192mM, in vitroand in vivo,respectively.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, University of Tokyo, Kongo, Bunkyo-ku, Tokyo, Japan

    Jiunn Huei Lin, Masahiro Hayashi, Shoji Awazu & Manabu Hanano

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  1. Jiunn Huei Lin
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  2. Masahiro Hayashi
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  3. Shoji Awazu
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  4. Manabu Hanano
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Lin, J.H., Hayashi, M., Awazu, S. et al. Correlation betweenin vitro andin vivo drug metabolism rate: Oxidation of ethoxybenzamide in rat. Journal of Pharmacokinetics and Biopharmaceutics 6, 327–337 (1978). https://doi.org/10.1007/BF01060096

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  • DOI: https://doi.org/10.1007/BF01060096

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