Abstract
99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.
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Xavier, C. et al. (2012). Site-Specific Labeling of His-Tagged Nanobodies with 99mTc: A Practical Guide. In: Saerens, D., Muyldermans, S. (eds) Single Domain Antibodies. Methods in Molecular Biology, vol 911. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-968-6_30
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DOI: https://doi.org/10.1007/978-1-61779-968-6_30
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-968-6
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