Regular Article
Proteome Analysis of Hepatocellular Carcinoma

https://doi.org/10.1006/bbrc.2002.6547Get rights and content

Abstract

Development of hepatocellular carcinoma (HCC) is a complex process involving multiple changes in gene expression and usually occurs in the presence of liver cirrhosis. In this research, we observed proteome alterations of three tissue types isolated from livers of HCC patients: normal, cirrhotic, and tumorous tissue. Proteome alterations were observed using two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Comparing the tissue types with each other, a significant change in expression level was found in 21 proteins. Of these proteins, sarcosine dehydrogenase, liver carboxylesterase, peptidyl-prolyl isomerase A, and lamin B1 are considered novel HCC marker candidates. In particular, lamin B1 may be considered as a marker for cirrhosis, because its expression level changes considerably in cirrhotic tissue compared with normal tissue. The proteins revealed in this experiment can be used in the future for studies pertaining to hepatocarcinogenesis, or as diagnostic markers and therapeutic targets for HCC.

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      Furthermore, increased levels of LMNB1 associated with larger tumors, increased number of tumor foci, and more advanced disease; LMNB1 mRNA was increased in plasma from patients with HCC compared to individuals without HCC.51 LMNB1 might therefore serve as a prognostic marker for patients with early- and late-stage HCC.83,84 Levels of lamin-associated and other nuclear proteins have also been examined in gastrointestinal tumors.

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    Abbreviations used: HCC, hepatocellular carcinoma; 2-DE, two-dimensional polyacrylamide gel electrophoresis; MALDI–TOF, matrix-assisted laser desorption/ionization time-of-flight; MS, mass spectrometry; HBV, hepatitis B virus; HCV, hepatitis C virus; IEF, isoelectric focusing; SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.

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