Regular Article
Cloning of the Human Sodium Iodide Symporter

https://doi.org/10.1006/bbrc.1996.1358Get rights and content

Abstract

The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland. An open reading frame of 1929 nucleotides encodes a protein of 643 amino acids with 84% identity to the rat NIS (rNIS). Transient expression of the hNIS cDNA conferred perchlorate-sensitive iodide uptake to a nonthyroid cell line, COS-7. The expression of hNIS was detected at variable levels in papillary thyroid carcinoma tissues but not in any of the thyroid carcinoma cell lines that have lost the iodide uptake activity.

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    After total thyroidectomy, the remaining NIS expression then allows for radioiodine ablation of metastases and residual thyroid tissue using 131I. After the identification of the NIS-encoding genes [9–11], iodide uptake could be evoked in non-thyroid cancer cells using gene therapy approaches. The resulting induced iodide uptake enabled noninvasive imaging of gene transfer monitoring and radioiodine ablation of targeted cells [12,13,14].

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The nucleotide sequence of the human sodium iodide symporter reported in this paper has been submitted to the Genbank database. The accession number assigned to this sequence is U66088.

Abbreviations used: hNIS, human sodium iodide symporter; rNIS, rat sodium iodide symporter; RT-PCR, reverse-transcription polymerase chain reaction.

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Correspondence to Dr. Sissy M. Jhiang, 302 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210. Fax: 614-292-4888.

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