Affinity of Human Antibody Lead as Recombinant scFv-CD44 or scFv-Fc-CD44
| Test | scFv-CD44 | scFv-Fc-CD44 |
| ELISA (n = 3) | ||
| EC50 ± SE (nM) | 26.3 ± 5.3 | 0.7 ± 0.1 |
| R2 | 0.927 | 0.923 |
| Flow cytometry (n = 3) | ||
| EC50 ± SE (nM) | 512.4 ± 50.5 | 2.3 ± 0.2 |
| R2 | 0.970 | 0.985 |
EC50 was calculated from equilibrium-binding curves toward recombinant human CD44 (amino acids 21–268) by enzyme-linked immunosorbent assay (ELISA) and by flow cytometry on MDA-MB-231 tumor cells.