m/z | |||
Compound | Calculated | Found | Chemical yield (%) |
Glu-urea-Lys-HBED-CC-FITC | 1,355.4 | 1,356.9 | 23 |
Glu-urea-Lys-HBED-CC-AlexaFluor488 | 1,478.4 | 1,481.5 | 27 |
Glu-urea-Lys-HBED-CC-IRDye800CW | 1,950.2 | 1,951.4 | 35 |
Glu-urea-Lys-HBED-CC-DyLight800 | 1,902.1 | 1,901.9 | 18 |
m/z = mass-to-charge ratio (g/mol) on mass spectrometry of nonlabeled ligand; FITC = fluorescein isothiocyanate.
Chemical yield refers to fluorescent dye conjugation reaction to Glu-urea-Lys[Fe(HBED-CC)]-PEG2-NH2.