Primers Used for PCR Genotyping
PCR reaction | Primer sequences |
5′ junction assay | CACTTTGAGCTCTACTGGCTTC |
CATGTTAGAAGACTTCCTCTGC | |
3′ junction assay | TTCACTGCATTCTAGTTGTGG |
AAGGCAGCCTGGTAGACA | |
5′ random integration | GTACTTTGGGGTTGTCCAG |
TTGTAAAACGACGGCCAG | |
3′ random integration | CCTGAGTTCTAACTTTGGCTC |
ACACAGGAAACAGCTATGAC | |
WT allele | TTCGGGTCACCTCTCACTCC |
GGCTCCATCGTAAGCAAACC |