Summary of Analytic Results for Compounds 1 and 2
| Purity determined by HPLC at: | |||||
| Compound | m/z calculated | m/z found | HPLC retention time (min) | 254 nm | 220 nm |
| 1 | 908.93 | 908.7 | 3.74 | >97.3% | >97.3% |
| 2 | 1,032.01 | 1,032.1 | 4.49 | >96.5% | >98.1% |
HPLC was performed with Merck Chromolith Performance RP-18e column (100–4.6 mm), gradient of 5% solvent A (acetonitrile)/95% solvent B (0.1% aqueous trifluoroacetic acid) to 95% A/5% B in 12.5 min, flow of 3 mL/min, and dead time of 0.56 min.