TABLE 1

Estimate of Absorbed Dose to N of MCF7/HER2-18 Cells Exposed to 64Cu-DOTA-Trastuzumab (0.245 MBq/μg, 67 nM) for 18 Hours, Followed by Clonogenic Assay

SourceÃs (Bq × s)S value (GyBq−1s−1)D (Gy)
18-h exposure
 Cell surface(2.87 ± 0.03) × 1044.04 × 10-5*1.16 ± 0.01
 Cytoplasm(6.2 ± 0.4) × 1035.89 × 10-5*0.36 ± 0.02
 Nucleus(4.4 ± 0.4) × 1032.55 × 1 0-4*1.10 ± 0.10
 Medium1.9 × 10114.16 × 10−120.8
10-d colony-formation
 Cell surface(5.3 ± 0.3) × 1032.96 × 10-5†0.157 ± 0.008
 Cytoplasm(1.12 ± 0.03) × 1034.81 × 10-5†0.054 ± 0.001
 Nucleus(5.9 ± 0.1) × 1022.44 × 10-4†0.145 ± 0.003
 Medium(6.3 ± 0.5) × 1054.16 × 10−12(2.6 ± 0.2) × 10−6
Total D (Gy)3.8 ± 0.1

  • ↵* Mean monolayer S values (N←CS, N←Cy, N←N), which is sum of respective self- and cross-dose S values.

  • ↵† Single cell S values (N←CS, N←Cy, N←N).

  • Ãs = time-integrated radioactivity in source compartment.