Binding Parameters of 125I-DRM106
| High-affinity binding site | Low-affinity binding site | |||||
| Sample | Kd* | Bmax† | BP‡ | Kd | Bmax | BP |
| Aβ(1–40) fibrils (n = 4) | 1.50 ± 0.00 | 4.30 ± 1.00 | — | 1,400 ± 36.6 | 163 ± 9.60 | — |
| Aβ(1–42) fibrils (n = 4) | 10.1 ± 5.10 | 34.3 ± 22.8 | — | 2,890 ± 344.5 | 339 ± 268 | — |
| Transgenic brain (n = 4) | 4.20 ± 0.92 | 113 ± 133 | 26.9 | 264 ± 160 | 3,780 ± 1,008 | 14.3 |
| AD brain | 4.30 ± 1.20 | 681 ± 305 | 158.4 | 101 ± 37.8 | 7,050 ± 1,985 | 69.8 |
↵* Kd is expressed as nmol/L.
↵† Bmax for Aβ fibrils and mouse/human tissues is expressed as pmol/nmol of Aβ and pmol/g of tissue.
↵‡ BP = Bmax/Kd.
Data of Aβ fibrils were from 4 independent experiments, and each experiment was run in triplicate. Data of transgenic brains were from 4 mice (20 mo old), and each experiment was run in triplicate. Data of AD brain were from triplicate experiments. Data are mean ± SD.
Bmax = maximum binding value; mo = month.