Dependence of Relative Advantage on Specific Activity When Cells Are Treated with Radiolabeled Ab2 vs. Cocktail of Radiolabeled Antibodies
Specific activity | |||
→→→increasing→→→ | |||
Relative advantage | A | B | C |
![]() | 1.26 ± 0.02 | 2.67 ± 0.44 | 1.38 ± 0.62 |
![]() | 2.20 ± 0.98 | 0.65 ± 0.25 | 0.54 ± 0.15 |
![]() | 2.59 ± 0.57 | 1.53 ± 1.04 | 1.13 ± 0.75 |
Specific activity of 211At-antibodies is 9.25 × 1013 Bq mol−1 for A, 9.25 × 1014 Bq mol−1 for B, and 9.25 × 1015 Bq mol−1 for C in experiment 2 and 1.25 × 1016 Bq mol−1 in experiment 1. Specific activity of 213Bi-antibodies is 1.16 × 1016 Bq mol−1 for A, 1.16 × 1017 Bq mol−1 for B, and 1.56 × 1017 Bq mol−1 for C. Specific activity of 225Ac-antibodies is 8.14 × 1012 Bq mol−1 for A, 8.14 × 1013 Bq mol−1 for B, and 1.14 × 1014 Bq mol−1 for C. Ratio > 1 implies cocktail is more lethal than single antibody. Ratios are average of 2 experiments. SEs are noted. Ab2 alone contains Ab2 (3 μg mL−1), cocktail contains Ab2 (1 μg mL−1), Ab3 (1 μg mL−1), and Ab4 (1 μg mL−1). Specific activity in column B is cited in literature for given radionuclide (4,21,22). Column A is 10-fold reduction of that specific activity, and column C is specific activity required to result in about 10 survivors of analyzed population of 300,000 cells.