TABLE 3

Summary of the reaction conditions for the radiosynthesis of [¹⁸F]FLT on EWOD chip based on Method 2.

Process	Temperature (°C)	Reaction time (min)	Reagent	Concentration (mM)	Droplet volume (μL)	Fluorination efficiency (%)^*	Radioactivity remaining on chip (%)^†	Crude radiochemical yield (%)^*
1—Load ¹⁸F-TBAF	Room temperature		TBAHCO₃	45	6	Not applicable		Not applicable
2—MeCN azeotropic distillation	105	2	MeCN	Not applicable	9	Not applicable		Not applicable
3—Fluorination	120	3	¹⁸F-FLT precursor	90	2	91 ± 4 (n = 10)	94 (n = 4)	Not applicable
4—Hydrolysis	95	1.5	HCl	75	7	Not applicable	85 (n = 4)	82 ± 10 (n = 11)

↵* Fluorination efficiencies and yields are reported with SD based on number of experiments, n.
↵† To determine radioactivity remaining on chip, chip was removed after each step and radioactivity on entire chip was measured using dose calibrator for radioactivity measurement.