RT-PCR Analysis of Multidrug Resistance and Multidrug Resistance Associated Protein Expression in Human Cancer Cell Lines
| MDR1/β2-m | MRP/β2-m | ||||
| Cell line | Basal* | BSO* | Basal* | BSO* | BSO/basal (%) |
| MCF7 | 0 | 0 | 0.22 | 0.43 | 195 |
| KB3.1 | 0 | 0 | 0.40 | 0.43 | 107 |
| H69 | 0 | 0 | 0.36 | 0.56 | 155 |
| KBA.1 | 2.6 | 2.6 | 0.34 | 0.48 | 141 |
| MCF7mdr+ | 1.15 | 1.3 | 0.59 | 0.70 | 118 |
| A549 | 0 | 0 | 0.57 | 0.64 | 112 |
| H69AR | 0 | 0 | 0.71 | 1.43 | 201 |
↵* ln cells treated, or not, with 25 μM BSO for 24 hr.
Results are given as ratio of MDR1or MRP and β2-microglobulin (β2-m) signals. RT-PCR = reverse transcriptase polymerase chain reaction; BSO = buthionine sulfoximine.