Analytic and Binding Data of Investigated Conjugates
| Compound | m/z* | m/z calculated as [M+H]+ | Cell surface bound (%ID/106 cells) | Internalized (%ID/106 cells) | Affinity-related IC50 (nM) determined in cell-based assay |
| Glu-urea-Lys(Ahx)-HBED-CC | 947.7 | 947.4 | 18.4 ± 1.8 | 4.8 ± 0.3 | 10.3 ± 1.2 |
| Glu-urea-Lys(Ahx)-HBED-CC-(EH)3 | 1,746.3 | 1,745.7 | 24.6 ± 2.7 | 6.7 ± 2.0 | 31.8 ± 1.2 |
| TATE-PEG2-HBED-CC | 1,709.0 | 1,708.7 | 6.9 ± 0.5 | 4.8 ± 0.3 | 45.1 ± 1.3 |
| TATE-PEG2-HBED-CC-(EH)3 | 2,507.2 | 2,507.0 | 4.8 ± 1.5 | 4.0 ± 1.3 | 48.9 ± 1.1 |
↵* Mass spectrometry data of free ligands ([M+H]+).
IC50 = 50% inhibitory concentration.