Quantitative Small-Animal PET Image Analyses Using DsRed-Positive LNs as True-Positive Indicator of LN Metastases
| LNs examined | TBR of %ID/g* | ||||||||
| mAb | No. of mice tested | n | Percentage DsRed-positive | Mean | SD | AUC† (%) | Sensitivity (%) | Specificity (%) | Optimal TBR cutoff (%ID/g) |
| 9601 | 10 | 136 | 11 | 3.68 | 3.2 | 60.7 | 66.7 | 70.3 | 2.81 |
| 153 | 9 | 125 | 14 | 3.77‡ | 0.9 | 78.0§ | 94.4 | 65.4 | 2.67 |
| 7 | 10 | 109 | 13 | 5.79‡ | 3.3 | 87.0§ | 92.9 | 67.4 | 2.44 |
| 69 | 15 | 167 | 10 | 1.79 | 0.9 | 27.0 | 43.8 | 38.4 | Not applicable∥ |
↵* Mean TBR (±SD) of %ID/g for DsRed-positive LNs in prostate cancer–positive mice.
↵† AUC from ROC curve analysis (curves are shown in Fig. 6).
↵‡ P < 0.05 for mAbs 7 and 153 vs. mAb 69 and for mAb 7 vs. mAb 9601. No significant difference was found between mAbs 7 and 153.
↵§ P < 0.001 for mAbs 7 and 153 vs. AUC of 50% (line of nondiscrimination).
↵∥ Not applicable because mAb 69 is isotype control.