TABLE 4

Biodistribution of ^99mTc-Z_HER2_:V1 and ^99mTc-Z_HER2_:V2 Affibody Molecules in Female BALB/c nu/nu Mice Bearing SKOV-3 Xenografts at 4 Hours After Intravenous Injection

	Uptake (%IA/g)		Tumor-to-organ ratio
Organ	^99mTc-Z_HER2_:V1	^99mTc-Z_HER2_:V2	^99mTc-Z_HER2_:V1	^99mTc-Z_HER2_:V2
Blood	0.29 ± 0.03^*	0.12 ± 0.01	76 ± 7^*	186 ± 29
Lung	0.46 ± 0.10	0.42 ± 0.08	49 ± 9	55 ± 11
Liver	1.4 ± 0.1^*	0.93 ± 0.09	15 ± 1^*	24 ± 3
Spleen	0.35 ± 0.06	0.42 ± 0.05	64 ± 11	53 ± 6
Stomach	0.4 ± 0.2	0.20 ± 0.07	50 ± 4^*	85 ± 7
Kidney	28.0 ± 2.0^*	8.2 ± 2.9	0.78 ± 0.06^*	2.9 ± 0.5
Salivary gland	0.23 ± 0.05^*	0.089 ± 0.005	98 ± 15^*	254 ± 45
Tumor	22.0 ± 3.3	22.6 ± 4.0
Muscle	0.08 ± 0.02	0.06 ± 0.04	296 ± 36	420 ± 152
Bone	0.29 ± 0.03^*	0.17 ± 0.04	75 ± 10^*	137 ± 15
Intestines^{^†}	2.4 ± 0.6	2.6 ± 0.9
Carcass^{^†}	2.9 ± 0.3^*	1.6 ± 0.1

↵* Significant difference between ^99mTc-Z_HER2_:V1 and ^99mTc-Z_HER2_:V2 Affibody molecules.
↵† Data for intestines with content and carcass are presented as percentage of injected radioactivity per whole sample.
%IA/g = percentage injected activity per gram of tissue.
Average tumor weight was 0.098 ± 0.04 g. Data are presented as average value from 4 animals ± SD.