Properties of Affibody Molecules
| Affibody molecule | C-terminus | Molecular weight (Da) | Isoelectric point | Melting point (°C) | On-rate ka (1/Ms) | Off-rate kd (1/s) | Dissociation constant KD (pM) |
| ZHER2:V1 | −GSEC | 6,758.5 | 6.42 | 63 | 3.29 × 106 | 4.68 × 10−4 | 142 |
| ZHER2:V2 | −GGGC | 6,656.4 | 8.14 | 65 | 4.25 × 106 | 4.87 × 10−4 | 150 |
| ZHER2:V3 | −GGSC | 6,696.5 | 8.14 | 66 | 2.81 × 106 | 5.29 × 10−4 | 188 |
| ZHER2:V4 | −GGEC | 6,728.5 | 6.42 | 65 | 1.94 × 106 | 4.95 × 10−4 | 255 |
| ZHER2:V5 | −GGKC | 6,727.5 | 8.91 | 66 | 4.06 × 106 | 6.51 × 10−4 | 160 |