Primers and Restriction Enzymes
| SNP | Primer sequence (5′→3′) | Template size (base pairs) |
| XbaI G>T | ||
| Restriction enzyme, XbaI | F-TGC AAC CCA TGA GCT AAC AA | 305 |
| R-GAA CCC AGC ACT CTG TAG CC | ||
| Restriction fragments | 232 | |
| 73 | ||
| HpyCH4V A>T | ||
| Restriction enzyme, HpyCH4V | F-GCT GAG AAT GGC CTT CCC TCA AT | 339 |
| R-GTC TGC CTT ACT CAG CCC ATG GGT C | ||
| Restriction fragments | 209 | |
| 130 | ||
| HaeIII T>C | ||
| Restriction enzyme, HaeIII | F-CTC CCA GAC ACG CCT ATA ACA GT | 173 |
| R-GGC TGG TGT CCA TAA GCC AAC G | ||
| Restriction fragments | 138 | |
| 35 |