Primer Sequences
| Primer | Sequence (5′ to 3′) | Genomic position | Product Size (bp) | Accession no. |
|---|---|---|---|---|
| β-actin F | CGACATGGAGAAGATCTGGC | 319–338 | 739 | NM_007393 |
| β-actin R | TCTTCATGGTGCTAGGAGCC | 1,039–1,058 | ||
| Luciferase F | CCTCATAGAACTGCCTGCG | 910–928 | 689 | U47296 |
| Luciferase R | AGACTTCAGGCGGTCAACGAT | 1,579–1,599 | ||
| hNIS F* | GCTGAGGACTTCTTCACCGGGGGCCG | 477–501 | 581 | U66088 |
| hNIS R* | GTCAGGGTTAAAGTCCATGAGGTTG | 1,034–1,058 |
↵* Pair of degenerated primers was designed by Huang et al (20).