Biodistribution of 99mTc-Radiolabeled UA20 (at 1 and 3 Hours After Injection) and Control N3M2 scFvs (at 3 Hours After Injection) in Nude Mice Bearing Prostate Tumor Xenografts (n = 4)
| UA20 | ||||
|---|---|---|---|---|
| Site | 1 h | 3 h | N3M2 | UA20 blocking |
| Tumor | 3.71 ± 0.23 | 4.40 ± 0.35 | 0.26 ± 0.11 | 1.40 ± 0.50 |
| Liver | 13.2 ± 1.14 | 2.74 ± 0.24 | 13.8 ± 1.65 | 2.50 ± 0.40 |
| Heart | 0.36 ± 0.08 | 0.13 ± 0.06 | 0.64 ± 0.13 | 0.20 ± 0.07 |
| Kidneys | 100 ± 9.42 | 81.4 ± 7.89 | 81.5 ± 8.23 | 101 ± 15.6 |
| Lung | 1.51 ± 0.17 | 0.57 ± 0.12 | 1.38 ± 0.21 | 1.21 ± 0.32 |
| Spleen | 5.44 ± 0.47 | 0.84 ± 0.11 | 6.68 ± 0.07 | 0.53 ± 0.32 |
| Stomach | 0.14 ± 0.02 | 0.64 ± 0.22 | 0.56 ± 0.19 | 0.81 ± 0.04 |
| Small intestine | 0.65 ± 0.07 | 0.80 ± 0.16 | 1.15 ± 0.28 | 0.54 ± 0.11 |
| Large intestine | 1.48 ± 0.23 | 0.81 ± 0.31 | 1.03 ± 0.25 | 0.63 ± 0.34 |
| Muscle | 0.13 ± 0.02 | 0.06 ± 0.01 | 0.35 ± 0.12 | 0.10 ± 0.04 |
| Fat | 0.11 ± 0.01 | 0.07 ± 0.01 | 0.23 ± 0.08 | 0.42 ± 0.30 |
| Blood | 0.68 ± 0.22 | 0.37 ± 0.14 | 1.97 ± 0.36 | 0.57 ± 0.24 |
Additional control (cold UA20 blocking) was also performed with 10-fold excess of cold UA20 scFv injected 1 h before injection of 99mTc-radiolabeled UA20. Biodistribution of 99mTc-radiolabeled UA20 at 3 h after injection was shown for this control experiment (UA20 blocking). Data are %ID/g ± SD.