TABLE 1

Biodistribution of ¹¹¹In-DOTA-Nle-CycMSH_hex in B16/F1 Melanoma–Bearing C57 Mice

	Biodistribution at…
Tissue	0.5 h	2 h	4 h	24 h	2-h NDP blockade	2-h L-lysine coinjection
%ID/g^*
Tumor	24.94 ± 4.58	19.39 ± 1.65	17.01 ± 2.54	10.53 ± 1.11	0.81 ± 0.03^†	14.48 ± 3.25
Brain	0.21 ± 0.07	0.02 ± 0.01	0.06 ± 0.03	0.03 ± 0.01	0.01 ± 0.01	0.04 ± 0.01
Blood	3.33 ± 0.35	0.11 ± 0.07	0.05 ± 0.02	0.02 ± 0.01	0.07 ± 0.05	0.92 ± 0.48
Heart	1.24 ± 0.15	0.16 ± 0.10	0.12 ± 0.03	0.07 ± 0.05	0.06 ± 0.02	0.37 ± 0.02
Lung	2.45 ± 0.83	0.32 ± 0.10	0.10 ± 0.05	0.10 ± 0.03	0.30 ± 0.06	0.75 ± 0.21
Liver	2.75 ± 0.26	1.46 ± 0.20	1.72 ± 0.07	1.89 ± 0.14	1.46 ± 0.08	1.42 ± 0.30
Spleen	1.09 ± 0.33	0.41 ± 0.13	0.47 ± 0.13	0.32 ± 0.08	0.44 ± 0.02	0.43 ± 0.07
Stomach	3.20 ± 0.98	1.25 ± 0.24	1.49 ± 0.12	1.34 ± 0.42	0.36 ± 0.14	1.64 ± 0.78
Kidneys	16.20 ± 4.32	9.52 ± 0.44	9.99 ± 1.39	9.31 ± 0.91	11.56 ± 0.56	6.66 ± 0.62^†
Muscle	0.60 ± 0.22	0.15 ± 0.08	0.10 ± 0.08	0.03 ± 0.01	0.02 ± 0.01	0.10 ± 0.08
Pancreas	1.18 ± 0.38	0.14 ± 0.02	0.16 ± 0.02	0.23 ± 0.08	0.12 ± 0.02	0.21 ± 0.05
Bone	1.34 ± 0.40	0.18 ± 0.10	0.22 ± 0.15	0.16 ± 0.03	0.05 ± 0.04	0.55 ± 0.14
Skin	4.11 ± 0.72	0.66 ± 0.23	0.53 ± 0.05	0.64 ± 0.16	0.29 ± 0.02	1.02 ± 0.09
%ID^*
Intestines	2.16 ± 0.28	1.40 ± 0.56	3.03 ± 1.06	1.41 ± 0.86	1.14 ± 0.47	1.85 ± 0.73
Urine	57.00 ± 3.91	82.23 ± 5.83	84.61 ± 5.21	87.29 ± 3.60	92.25 ± 1.56	76.79 ± 5.35
Tumor–to–normal-tissue uptake ratio
Tumor-to-blood	7.49	176.27	340.20	526.50	11.57	15.74
Tumor-to-kidneys	1.54	2.04	1.70	1.13	0.07	2.17
Tumor-to-lung	10.18	60.59	170.10	105.30	2.70	19.31
Tumor-to-liver	9.07	13.28	9.89	5.57	0.55	10.20
Tumor-to-muscle	41.57	129.27	170.10	351.00	40.50	144.80
Tumor-to-skin	6.07	29.38	32.09	16.45	2.79	14.20

↵* Mean ± SD (n = 5).
↵† P < 0.05, significance comparison between tumor uptake of ¹¹¹In-DOTA-Nle-CycMSH_hex with or without NDP-MSH blockade and kidney uptake of ¹¹¹In-DOTA-Nle-CycMSH_hex with or without L-lysine coinjection.
Data are presented as percent %ID/g or as %ID (mean ± SD, n = 5).