TABLE 2

Experimental Protocols and Conclusions

Exposure [model]	AA5 and tracer	Time of injection	Ischemia (min)	Reperfusion (min)	Conclusion
^99mTc-AA5–based imaging of externalized PS in ischemic myocardium [rabbit]
1	^99mTc-AA5	At reperfusion	10	180 min	Brief ischemia results in detectable PS exposure but no apoptosis or necrosis seen histopathologically
2	^99mTc-AA5	At reperfusion	40	180 min	Prolonged ischemia results in detectable PS exposure, apoptosis, and necrosis
Cardiomyocytes expose PS after brief ischemia [mouse]
1	AA5	Before ischemia	5	90 min, 24 h	Brief ischemia results in PS exposure but no TUNEL positivity
2	AA5	Before ischemia	30	90 min, 24 h	Prolonged ischemia results in PS exposure and TUNEL positivity
PS and AA5 are internalized in cardiomyocytes [mouse]
1	AA5 Oregon Green	At reperfusion	5	90 min	Brief ischemia results in PS exposure on cardiomyocytes
	AA5 Alexa 568	30 min before sacrifice			that bind and internalize AA5
Characterization of PS-dependent internalization [mouse]
1	AA5 M23-Alexa 568	Before ischemia	5	90 min	2-dimensional crystallization of AA5 is mandatory for internalization
2	AA5 M1234-FITC	Before ischemia	5	90 min	PS binding of AA5 is mandatory for internalization
Cardiomyocytes expose PS up to 6 h after brief ischemia [mouse]
1	AA5-biotin	10 min before sacrifice	5	90 min, 3 h, 6 h, 24 h	Brief ischemia results in caspase-dependent PS exposure
PS exposure is a transient process [mouse]
1	AA5 Oregon Green	At reperfusion	5	90 min, 6 h, 24 h	Brief ischemia results in PS exposure that is transient and persists up to 6 h
	AA5 Alexa 568	10 min before sacrifice

PS = phosphatidylserine.
M23 and M1234 are AA5 mutants.