Cell Viability Test at Various Times After PDT
| Time after PDT (min) | Dish no. | Total | Viable | Nonviable | Nonviable % |
|---|---|---|---|---|---|
| 30 | 1 | 200 | 193 | 7 | 3.5% |
| 2 | 200 | 198 | 2 | 1.0% | |
| 3 | 200 | 196 | 4 | 2.0% | |
| 45 | 4 | 224 | 217 | 7 | 3.1% |
| 5 | 235 | 225 | 10 | 4.3% | |
| 6 | 452 | 430 | 22 | 4.9% | |
| 60 | 7 | 208 | 203 | 5 | 2.4% |
| 8 | 250 | 244 | 6 | 2.4% | |
| 9 | 273 | 268 | 5 | 1.8% | |
| 450 | 10 | 227 | 210 | 17 | 7.5% |
| 11 | 230 | 212 | 18 | 7.8% | |
| 12 | 213 | 193 | 20 | 9.4% | |
| No PDT450 | 13 | 211 | 207 | 4 | 1.9% |
| 14 | 322 | 315 | 7 | 2.2% | |
| 15 | 203 | 198 | 5 | 2.5% |
Cell cultures were stained with trypan blue solution. Unstained (viable) and stained (nonviable) cells were counted for each dish (nos. 1–15). Percentages of nonviable cells were calculated for both treated and control dishes.