Results from In-Line Radiolabeling of 1 g of Antibody with Approximately 481 GBq (∼13 Ci) of 131I Using the Continuous-Flow Procedure
| Parameter | Day 0 | Day 3 | Day 3 + 6 h* | Day 3 + 24 h* | Day 3 + 48 h* | Day 5 |
|---|---|---|---|---|---|---|
| Percentage potency | † | † | 87 | 91 | 79 | 88 |
| Percentage monomer | >95 | 96.1 | 95.6 | >95 | 96.9 | 96.3 |
| Free iodine | 0.7 | 3.0 | 4.1 | 5.6 | 7.4 | 2.8 |
↵* Samples thawed on day 3 were also tested after storage at room temperature for 6, 24, and 48 h.
↵† Results had to be dropped, because linear regression analysis was not possible.
Reactant 1: 487.3 GBq (13,170 mCi) of Na[131I]I in 10 mL of 0.1 M phosphate buffer, pH 7.4; reactant 2: 30 mg of chloramine-T in 10 mL of H2O; reactant 3: 1,000 mg of Lym-1 in 50 mL of phosphate buffer; reaction tube volume: 23-mL silicon tube; flow rate: 2.3 mL/min; total reaction time: 30 min; yield: 466.8 GBq (12,616 mCi) → 95.6%.