TABLE 1

Primer and Probe Sequences and Amplicon Length for Investigated Genes

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	5′ fluorophore	Probe (5′-3′)	3′ quencher	Amplicon length
TBP	tccttcaccaatgactcctatgac	tcaagtttacagccaagattcacg	HEX	cctgccacaccagcctctgagagc	BHQ-1	120 bp
RPL13	tcgtgaggtgccctacagttag	ggtgcgtgccattttcttgtg	HEX	cacaccaaggtccgggctggcag	BHQ-1	107 bp
Muscle HIF1α	ggattccagcagacccagttac	gggtagaaggtggagatgaatc	FAM	accatcactgtcactgccaccgca	BHQ-1	139 bp
Tendon HIF1α	caagcagcaggaattggaacg	tgctccattccatcctgttcac	FAM	tgcagcaaccaggtgaccgtgcc	BHQ-1	116 bp
Muscle CAIII	aggctccttttaatcacttcgac	ctttcagtagcagccacacaatg	CY5	cgtgcctgttccctgcttgccgg	BHQ-2	123 bp
Tendon CAIII	gaggctctccttttaatcacttcga	tcactgtcatgggctctttcag	CY5	acaatgcactcctcgcagggtggc	BHQ-2	139 bp