Kinetic Constants of 19G9 IgG Antibody and Fragment Antibody Formats Measured by SPR
| Constant | ||||
|---|---|---|---|---|
| Antibody format | ka (1/mol s) | kd (1/s) | KA (1/M) | KD (M) |
| scFv 19G9 | 9.0 × 104 | 1.7 × 10−3 | 5.3 × 107 | 1.9 × 10−8 |
| Diabody 19G9 | 1.85 × 105 | 2.14 × 10−4 | 9.0 × 108 | 1.2 × 10−9 |
| Miniantibody 19G9 | 7.6 × 105 | 1.14 × 10−4 | 6.7 × 108 | 2.0 × 10−9 |
| IgG 19G9 | 1.1 × 105 | 1.1 × 10−4 | 9.9 × 108 | 1.2 × 10−9 |