TABLE 1

Biodistribution of ¹¹¹In-Labeled Tracers (Injected Dose, 3 μg) 4 and 24 Hours After Injection in BALB/c nu/nu Mice Bearing EGFR-Expressing A431 Xenografts

	4 h after injection				24 h after injection
Organ or tissue	¹¹¹In-Bz-DTPA-Z_EGFR:1907	¹¹¹In-Bz-DTPA-Z_Taq	¹¹¹In-Bz-DTPA-(Z_EGFR:1907)₂	¹¹¹In-Bz-DTPA-(Z_Aβ)₂	¹¹¹In-Bz-DTPA-Z_EGFR:1907	¹¹¹In-Bz-DTPA-(Z_EGFR:1907)₂
Blood	1.5 ± 0.5^*	0.03 ± 0.01	1.5 ± 0.7	0.037 ± 0.002	0.07 ± 0.02^*	0.07 ± 0.02^†
Lung	1.3 ± 0.2^*^‡	0.25 ± 0.04	1.1 ± 0.2^†	0.15 ± 0.01	0.28 ± 0.04^*^‡	0.5 ± 0.2^†
Liver	5 ± 1^*^‡	1.4 ± 0.2	18 ± 5^†	0.6 ± 0.2	1.2 ± 0.3^*^‡	6 ± 1^†^‡
Spleen	0.7 ± 0.2^*	0.41 ± 0.06	1.1 ± 0.2^†	0.18 ± 0.03	0.33 ± 0.04^*	0.76 ± 0.05^†
Colon	1.2 ± 0.2^*	0.30 ± 0.05	1.6 ± 0.5	0.23 ± 0.05	0.44 ± 0.08^*	1.35 ± 0.09^†
Kidney	136 ± 11^*^‡	189 ± 19	73 ± 7^†	207 ± 35	102 ± 2^*	59 ± 7^†
Tumor	3.73 ± 0.08^*^‡	0.4 ± 0.2	1.57 ± 0.07	0.19 ± 0.07	2.0 ± 0.3^*^‡	1.23 ± 0.07^†
Muscle	0.29 ± 0.06^*	0.06 ± 0.01	0.24 ± 0.07	0.06 ± 0.04	0.09 ± 0.02^*	0.20 ± 0.05^†
Bone	0.5 ± 0.3	0.3 ± 0.2^§	0.7 ± 0.2	0.16 ± 0.10	0.3 ± 0.2^‡	0.6 ± 0.2^†
Brain	0.06 ± 0.02^*	0.004 ± 0.002	0.06 ± 0.03	NM	0.01 ± 0.01^‡	0.04 ± 0.03
GI tract^‖	3.6 ± 0.8^*^‡	1.0 ± 0.4	6.0 ± 0.7^†	1.0 ± 0.3	1.2 ± 0.2^*^‡	6 ± 1^†
Carcass^‖	13 ± 1^*	3 ± 1	12 ± 2^†	1.8 ± 0.3	4.9 ± 0.5^‡	9.6 ± 0.5^†

↵* Significant difference (P < 0.05, paired t test) between ¹¹¹In-Bz-DTPA-Z_EGFR:1907 and ¹²⁵I-PIB-Z_EGFR:1907.
↵† Significant difference (P < 0.05, paired t test) between ¹¹¹In-Bz-DTPA-(Z_EGFR:1907)₂ and ¹²⁵I-PIB-(Z_EGFR:1907)₂.
↵‡ Significant difference (P < 0.05, unpaired t test) between ¹¹¹In-Bz-DTPA-Z_EGFR:1907 and ¹¹¹In-Bz-DTPA- (Z_EGFR:1907)₂.
↵§ No significant difference (P > 0.05, unpaired t test) between ¹¹¹In-Bz-DTPA-Z_EGFR:1907 and ¹¹¹In-Bz-DTPA-Z_Taq.
↵‖ Data for gastrointestinal (GI) tract and carcass are presented as %IA per sample.
NM = nonmeasureable.
Data are presented as average %IA/g and SD (n = 4). Nonspecific Affibody molecules Z_Taq and (Z_Aβ)₂ have been included as negative control for ¹¹¹In-Bz-DTPA-Z_EGFR:1907 and ¹¹¹In-Bz-DTPA-(Z_EGFR:1907)₂, respectively.