D0 and Lower Asymptote for Clonogenic Survival of HL-60 and Mitoxantrone-Resistant HL-60-MX-1 Cells Exposed to Radioconjugates at Different Specific Activities
| HL-60 | HL-60-MX-1 | |||
|---|---|---|---|---|
| Radioimmunoconjugate and specific activity (MBq/μg) | D0 (mBq/cell) | Lower asymptote (% cells surviving) | D0 (mBq/cell) | Lower asymptote (% cells surviving) |
| 111In-M195 | ||||
| 3 | 17.1 ± 3.6 | 53 ± 4 | ND | ND |
| 6 | 19.5 ± 2.1 | 53 ± 2 | 20.2 ± 2.6 | 55 ± 2 |
| 8 | 9.3 ± 1.3* | 61 ± 2* | 16.4 ± 2.4* | 55 ± 2* |
| 111In-NLS-M195 | ||||
| 3 | 25.3 ± 4.0 | 56 ± 3 | 24.3 ± 2.6 | 55 ± 1 |
| 6 | 16.7 ± 2.3 | 50 ± 5 | 13.5 ± 1.1 | 34 ± 1 |
| 8 | 9.8 ± 0.8* | 43 ± 1* | 15.8 ± 0.5* | 38 ± 1* |
| 111In-NLS-HuM195 | ||||
| 3 | 23.8 ± 4.9 | 67 ± 4 | ND | ND |
| 6 | 22.5 ± 4.3 | 60 ± 3 | 16.4 ± 3.0 | 54 ± 4 |
| 8 | 5.6 ± 0.6* | 42 ± 1* | 16.1 ± 1.9* | 44 ± 4* |
↵* Significantly different values (P < 0.05).
Values shown are mean ± SD of triplicate determinations. No effects on clonogenic survival of HL-60 or HL-60-MX-1 cells were observed for 111In-labeled mAbs at 1 MBq/μg (not shown). ND = not determined.