TABLE 1

mRNA Expression of PEPT1 and PEPT2 in Various Human Cancer Cell Lines

Source tissue & cell namePEPT1PEPT2Source tissue & cell namePEPT1PEPT2Source tissue & cell namePEPT1PEPT2
LungBladderBrain
 A549UQL39 ± 10 T240.2 ± 0.02 ± 0 T98GUQLUQL
 PC-10UQL53 ± 2Uterus CCF-STTG1UQL11 ± 5
 Lu99BUQL23 ± 5 JHUEM-7UQL12 ± 4 IMR-32UQL82 ± 31
 T3 M-12UQL29 ± 27 JHUCS-31.5 ± 0.041 ± 15 SCCH-26UQL166 ± 0
 EBC-1UQL76 ± 15CervixPleural effusion
 LK-21.1 ± 0.219 ± 10 HeLaUQL29 ± 6 U-937UQL333 ± 99
KidneyOvaryMalignant pleural mesothelioma
 ACHNUQL29 ± 2 JHOS-263.2 ± 8.71,887 ± 429 ACC-MESO-1UQL151 ± 53
 VMRC-RCW279.4 ± 72.735 ± 11 JHOS-40.5 ± 0.1361 ± 86Peripheral blood
 FU-RPNT-2UQL3,118 ± 16 JHOS-829.1 ± 3.3UQL PCM60.8 ± 0.4188 ± 6
Pancreas JHOM-2B1.3 ± 0.252 ± 21Blood
 PK-45-HUQLUQL KGNUQL97 ± 46 EoL-1UQL577 ± 129
 PK-1UQL1,550 ± 9Connective tissue KU812FUQL248 ± 55
 AsPC-1105.3 ± 51.393 ± 4 HT1080UQL8 ± 1 OIH-1UQL406 ± 7
StomachEpidermis HL-60UQL125 ± 36
 MKN4540.7 ± 4.43 ± 2 A431UQL3,037 ± 131 THP-1UQL278 ± 124
 LMSUUQLUQLEsophagus JurkatUQL118 ± 33
 AZ521UQL4 ± 0 TE-15UQL49 ± 7 MOLT-4UQL59 ± 12
 KATO III0.1 ± 0.010 ± 1 TE-5UQL162 ± 79Bone marrow
Liver TE-9UQL3,640 ± 364 HYT-1UQL1,392 ± 708
 HepG2UQL24 ± 3ThyroidBone
 HuH-6UQL15 ± 4 HTC/C31.4 ± 0.376 ± 22 MG-63UQL22 ± 7
Bile ductOral, thoracic cavity metaSkin
 HuCCT17.7 ± 1.643 ± 12 CJMUQL608 ± 224 SK-Mel-28UQL2 ± 0
Mammary glandGingiva
 T47-DUQL1 ± 0 Ca9-22UQL842 ± 376
 CRL1500UQL432 ± 2Tongue
Prostate HSC-30.5 ± 0.63,556 ± 1,534
 PC-30.1 ± 0.011 ± 2
  • UQL = under quantification limit (>0.05 copy/ng total RNA for PEPT1; >0.5 copy/ng total RNA for PEPT2).

  • Expression levels are reported as copy/ng total RNA (mean ± SD; n = 4). Determinations were performed in duplicate on each sample by 2-step real-time quantitative PCR using complementary DNA obtained by reverse transcription from total RNA of human cancer cell lines.