Biodistribution of [99mTc]Demobesin 4 in Human PC3 Xenograft–Bearing SCID Mice at 1, 4, and 24 Hours After Injection
| [99mTc]Demobesin 4 | |||
|---|---|---|---|
| Organ | 1 h | 4 h | 24 h |
| Blood | 1.40 ± 0.51 | 0.07 ± 0.01 | 0.07 ± 0.02 |
| Liver | 1.26 ± 0.35 | 0.63 ± 0.09 | 0.32 ± 0.02 |
| Heart | 0.68 ± 0.24 | 0.19 ± 0.03 | 0.08 ± 0.01 |
| Kidneys | 12.84 ± 3.61 | 7.41 ± 1.16 | 1.83 ± 0.27 |
| Stomach | 0.96 ± 0.44 | 0.34 ± 0.06 | 0.38 ± 0.01 |
| Intestines | 5.20 ± 1.51 | 5.79 ± 0.74 | 1.06 ± 0.02 |
| Blocked intestines*† | ND | 1.23 ± 0.21‡ | ND |
| Spleen | 2.23 ± 0.53 | 1.85 ± 0.56 | 0.84 ± 0.59 |
| Muscle | 0.31 ± 0.10 | 0.03 ± 0.00 | 0.06 ± 0.02 |
| Lungs | 1.33 ± 0.27 | 0.27 ± 0.05 | 0.25 ± 0.13 |
| Pancreas | 38.90 ± 8.41 | 34.80 ± 4.75 | 14.43 ± 2.65 |
| Blocked pancreas*† | ND | 0.68 ± 0.04‡ | ND |
| PC3 tumor | 9.37 ± 2.52 | 5.19 ± 0.59 | 2.67 ± 0.29 |
| Blocked tumor*† | ND | 0.55 ± 0.4‡ | ND |
| Tumor/blood | 6.69 | 74.14 | 38.14 |
| Tumor/muscle | 30.23 | 173.00 | 44.50 |
| Tumor/liver | 7.44 | 8.24 | 8.34 |
| Tumor/kidney | 0.73 | 0.70 | 1.46 |
↵* In vivo blockade was achieved by coinjection of 100 μg [Tyr4]bombesin.
↵† Statistical analysis was performed using the Student t test with P values indicating extremely significant difference (‡P < 0.001) vs. unblocked.
ND = not determined.
Data are expressed as ID%/g (percentage injected dose per gram) and are presented as mean ± SD (n = 4).