TABLE 1

Mass Spectrometric, RP-HPLC, and In Vitro IC50 Characterization of Conjugates

AnalogMolecular formulaMS calculatedMS observedRP-HPLC tr (min)*IC50 (nM)†
DOTA-8-AOC-BBN(7–14)NH2C67H106N18O17S1,467.81,468.013.8
Cu-DOTA-8-AOC-BBN(7–14)NH2C67H104N18O17SCu1,528.71,528.515.01.44 ± 0.08
CB-TE2A-8-AOC-BBN(7–14)NH2C67H108N18O13S1,405.81,405.815.80.67 ± 0.07
Cu-CB-TE2A-8-AOC-BBN(7–14)NH2C67H106N18O13SCu1,465.71,465.717.20.48 ± 0.01

  • ↵* RP-HPLC methods described in Materials and Methods.

  • ↵† In vitro binding assays were performed in triplicate.