Primers and Probes Used in Real-Time Polymerase Chain Reactions
| Parameter | Sequence |
|---|---|
| Glut-1 | |
| Forward | 5′-GCA ACG CCT GCT ACA TGG A-3′ |
| Reverse | 5′-GAT ACA CAT CCG CCC CTC AT-3′ |
| Probe | 5′-CGC CAC ATC GAC ATG GTG GAA GG-3′ |
| HKII | |
| Forward | 5′-CAT CGT CGT CGG CAT CCT-3′ |
| Reverse | 5′-CCT TGT TGC CCA TGA TGG A-3′ |
| Probe | 5′-ATC GCC CAG GTG TTC GGC CTG-3′ |
| β-actin | |
| Forward | 5′-TCA CCC ACA CTG TGC CCA TCT ACG A-3′ |
| Reverse | 5′-TCG GTG AGG ATC TTC ATG AGG TA-3′ |
| Probe | 5′-ATG CCC TCC CCC ATG CCA TCC-3′ |