Biodistribution of TTA1 and Control (TTA1.NB) Aptamers
| TTA1 | TTA1.NB | ||||
|---|---|---|---|---|---|
| Tissue | Time* (min) | Mean (±SE) %ID/g | Tumor-to-tissue ratio | Mean (±SE) %ID/g | Tumor-to-tissue ratio |
| Tumor | 2 | 4.47 ± 0.41 | 1.0 | 4.51 ± 0.30 | 1.0 |
| 10 | 5.94 ± 0.59 | 1.0 | 3.02 ± 0.21 | 1.0 | |
| 60 | 2.69 ± 0.31 | 1.0 | 0.15 ± 0.02 | 1.0 | |
| 180 | 1.88 ± 0.10 | 1.0 | 0.04 ± 0.00 | 1.0 | |
| 570 | 1.20 ± 0.07 | 1.0 | 0.02 ± 0.00 | 1.0 | |
| 1,020 | 1.15 ± 0.06 | 1.0 | NA | ||
| Blood | 2 | 18.25 ± 1.14 | 0.2 | 15.01 ± 0.51 | 0.3 |
| 10 | 2.27 ± 0.25 | 2.6 | 2.05 ± 0.20 | 1.5 | |
| 60 | 0.11 ± 0.00 | 24.0 | 0.10 ± 0.02 | 1.4 | |
| 180 | 0.03 ± 0.00 | 58.8 | 0.03 ± 0.00 | 1.3 | |
| 570 | 0.01 ± 0.00 | 92.2 | 0.01 ± 0.00 | 1.6 | |
| 1,020 | 0.01 ± 0.00 | 202.5 | NA | ||
| Lung | 2 | 8.97 ± 1.21 | 0.5 | 8.13 ± 0.96 | 0.6 |
| 10 | 2.13 ± 0.08 | 2.8 | 1.94 ± 0.23 | 1.6 | |
| 60 | 0.16 ± 0.01 | 17.1 | 0.12 ± 0.01 | 1.2 | |
| 180 | 0.05 ± 0.01 | 39.2 | 0.04 ± 0.00 | 1.0 | |
| 570 | 0.03 ± 0.01 | 42.8 | 0.02 ± 0.00 | 1.1 | |
| 1,020 | 0.01 ± 0.00 | 173.9 | NA | ||
| Liver | 2 | 9.12 ± 0.53 | 0.5 | 7.90 ± 0.35 | 0.6 |
| 10 | 12.46 ± 1.25 | 0.5 | 9.10 ± 0.83 | 0.3 | |
| 60 | 1.23 ± 0.09 | 2.2 | 0.42 ± 0.10 | 0.3 | |
| 180 | 0.40 ± 0.08 | 4.7 | 0.21 ± 0.06 | 0.2 | |
| 570 | 0.10 ± 0.02 | 11.5 | 0.06 ± 0.00 | 0.3 | |
| 1,020 | 0.07 ± 0.00 | 15.4 | NA | ||
| Spleen | 2 | 5.10 ± 0.41 | 0.9 | 4.86 ± 0.13 | 0.9 |
| 10 | 2.46 ± 0.21 | 2.4 | 1.22 ± 0.12 | 2.5 | |
| 60 | 0.64 ± 0.08 | 4.2 | 0.11 ± 0.02 | 1.3 | |
| 180 | 0.20 ± 0.03 | 9.5 | 0.04 ± 0.01 | 1.1 | |
| 570 | 0.06 ± 0.00 | 19.3 | 0.02 ± 0.00 | 0.9 | |
| 1,020 | 0.03 ± 0.00 | 38.7 | NA | ||
| Kidney | 2 | 44.43 ± 4.28 | 0.1 | 54.47 ± 1.21 | 0.1 |
| 10 | 18.81 ± 0.94 | 0.3 | 14.32 ± 2.08 | 0.2 | |
| 60 | 1.51 ± 0.04 | 1.8 | 0.64 ± 0.11 | 0.2 | |
| 180 | 0.29 ± 0.03 | 6.6 | 0.22 ± 0.02 | 0.2 | |
| 570 | 0.14 ± 0.01 | 8.6 | 0.10 ± 0.01 | 0.2 | |
| 1,020 | 0.08 ± 0.01 | 14.1 | NA | ||
| Sm. int. | 2 | 3.69 ± 0.25 | 1.2 | 3.12 ± 0.10 | 1.4 |
| 10 | 7.01 ± 0.07 | 0.8 | 6.44 ± 0.25 | 0.5 | |
| 60 | 15.72 ± 2.04 | 0.2 | 14.65 ± 0.53 | 0.0 | |
| 180 | 1.48 ± 0.71 | 1.3 | 1.24 ± 0.41 | 0.0 | |
| 570 | 0.22 ± 0.15 | 5.5 | 0.16 ± 0.07 | 0.1 | |
| 1,020 | 0.28 ± 0.24 | 4.1 | NA | ||
| Lg. int. | 2 | 2.34 ± 0.24 | 1.9 | 2.28 ± 0.18 | 2.0 |
| 10 | 0.89 ± 0.04 | 6.7 | 0.77 ± 0.07 | 3.9 | |
| 60 | 10.80 ± 5.38 | 0.2 | 21.66 ± 11.68 | 0.0 | |
| 180 | 26.18 ± 7.84 | 0.1 | 18.02 ± 3.49 | 0.0 | |
| 570 | 1.26 ± 0.71 | 0.9 | 0.72 ± 0.18 | 0.0 | |
| 1,020 | 0.30 ± 0.17 | 3.9 | NA | ||
| Muscle | 2 | 1.27 ± 0.13 | 3.5 | 1.49 ± 0.05 | 3.0 |
| 10 | 0.87 ± 0.09 | 6.8 | 1.84 ± 1.00 | 1.6 | |
| 60 | 0.06 ± 0.00 | 42.0 | 0.05 ± 0.00 | 2.9 | |
| 180 | 0.02 ± 0.00 | 117.7 | 0.01 ± 0.00 | 3.9 | |
| 570 | 0.01 ± 0.00 | 109.0 | 0.01 ± 0.00 | 2.6 | |
| 1,020 | 0.00 ± 0.00 | 420.3 | NA | ||
↵* Time at which animals were anesthetized, blood was collected, animals were killed, tissues were weighed, and radioactivity was quantified in γ-counter. n = 3 mice per interval.
Sm. int.= small intestine; Lg. int. = large intestine.