Extraction Efficiency and Elution Efficiency Data and HPLC Analysis of Soluble Fraction of Tissue Samples at 60 Minutes After Injection
| Fraction | Blood | Urine | Liver | Kidney | U87MG |
|---|---|---|---|---|---|
| Extraction efficiency (%) | |||||
| Unsoluble fraction* | 6.9 | ND | 12.6 | 16.5 | 21.7 |
| Soluble fraction† | 93.1 | ND | 87.4 | 83.5 | 78.3 |
| Elution efficiency (%) | |||||
| Nonretained fraction‡ | 2.1 | 0.2 | 37.1 | 14.5 | 15.2 |
| Wash water§ | 0.6 | 0.7 | 3.0 | 1.6 | 3.0 |
| Acetonitrile eluent¶ | 97.2 | 99.1 | 59.9 | 83.8 | 81.8 |
| HPLC analysis (%) | |||||
| Intact tracer | 90.6 | 95.5 | 93.5 | 87.8 | 79.9 |
↵* Amount of activity that was retained in pellets.
↵† Amount of activity that was extracted to PBS solution.
↵‡ Amount of activity that could not be trapped on C18 cartridge.
↵§ Amount of activity that was eluted from C18 cartridge using 2 mL water.
↵¶ Amount of activity that was eluted from C18 cartridge using 2 mL acetonitrile with 0.1% TFA.
ND = not determined.