Comparative HPLC Profiles of Peptide Conjugates and Their Metal Complexes
| Peptide | UV/Vis detection tR (min) | γ-Detection tR (min) | ||||
|---|---|---|---|---|---|---|
| System 1 | System 2 | System 1 | System 2 | |||
| Z-070 | ND | 18.3 | ND | ND | ||
| [111In]Z-070 | ND | ND | ND | 18.4 | ||
| [InIII]Z-070 | ND | 18.4 | ND | ND | ||
| [YIII]Z-070 | ND | 18.4 | ND | ND | ||
| [LuIII]Z-070 | ND | 18.4 | ND | ND | ||
| Demobesin 1 | 12.3 | 16.3 | ND | ND | ||
| [99mTc]Demobesin 1 | ND | ND | 14.3 | ND | ||
| [ReV]Demobesin 1 | 13.8 | 19.2 | ND | ND | ||
UV/Vis = UV visible; ND = not done.
Runs were conducted on a Waters XTerra RP-8 cartridge column (5 μm, 150 × 4.6 mm) at 1-mL/min flow rate adopting a linear gradient system starting from 15% solvent A (acetonitrile) and 85% solvent B (0.1% TFA in water) and reaching 75% solvent A and 25% solvent B within 30 min for system 1 and 60 min for system 2.