DOTA-α-MSH analog | Hydrophobicity (% acetonitrile at elution)* | Net charge at neutral pH † |
---|---|---|
DOTA-NAPamide | 48.29 ± 0.00 | +1 |
III | 48.25 ± 0.09 | 0 |
IV | 44.46 ± 0.12 | +2 |
V | 46.20 ± 0.07 | +1 |
VI | 46.3 ± 0.04 | −1 |
VII | 45.67 ± 0.22 | 0 |
↵* Hydrophobicity was assessed by RP-HPLC with increasing percentages of acetonitrile in water and 0.1% TFA as eluent. Results are expressed as percent acetonitrile (mean ± SD) necessary to elute DOTA-α-MSH analogs.
↵† Calculated net charge at pH 7 of 111ln-DOTA-α-MSH analogs based on pKa values. Note that charge variation is entirely dependent on C-terminal lysine residue upon conjugation of various charged or uncharged groups.