FL, RL, D2R, and TK Enzyme Activities in N2a Cells Transiently Transfected with Different Bicistronic Constructs Carrying EMCV IRES and SIRES Modules
| Plasmid | pGL3 backbone | TK | D2R | RL | FL |
|---|---|---|---|---|---|
| 1 | SV40-rl-IRES-tk (RIT) | 0.028 | 8.8 | ||
| 2 | SV40-rl-SIRES-tk (RST) | 0.339 | 58.7 | ||
| 3 | SV40-fl-IRES-tk (FIT) | 0.015 | 50.3 | ||
| 4 | SV40-fl-SIRES-tk (FST) | 0.234 | 96.9 | ||
| 5 | SV40-D2R-IRES-tk (DIT) | 0.03 | 0.3 | ||
| 6 | SV40-D2R-SIRES-tk (DST) | 0.343 | 0.5 | ||
| 7 | SV40-tk | 0.165 | |||
| 8 | SV40-fl | 62.4 | |||
| 9 | SV40-rl | 21.3 | |||
| 10 | SV40-D2R | 0.2 |
Plasmids SV40-tk, SV40-D2R, SV40-rl, and SV40-fl were used as control vectors in transfections. FL and RL activities are expressed as RLU/μg protein. Units of D2R activity are pmol 3H-spiperone bound/μg protein. TK activity is in units of dpm/mg protein/min. All enzyme activities were normalized to β-gal activity in each sample.