Hypoxic condition | Bmax (ng/dish) | Cell counts (×106/dish) | Bmax/cell (ng/106) | Binding sites (×105/cell) | Binding sites at 3 h after reoxygenation |
---|---|---|---|---|---|
Control | 67.1 ± 1.4 | 0.87 ± 0.08 | 77.2 ± 6.3 | 6.13 ± 0.50 | 6.39 ± 0.45 |
H-1 | 52.5 ± 2.1 | 0.72 ± 0.07 | 73.1 ± 8.3 | 5.80 ± 0.66 | 6.29 ± 0.54 |
H-2 | 34.5 ± 1.6 | 0.83 ± 0.07 | 41.8 ± 3.3 | 3.31 ± 0.26 | 5.97 ± 0.53 |
H-3 | 12.7 ± 0.8 | 0.80 ± 0.12 | 15.9 ± 1.7 | 1.27 ± 0.15 | 3.42 ± 0.40 |
F ratio | 691.6 | 1.6 | 81.3 | 80.7 | 25.5 |
P | <0.0001 | 0.27 | <0.0001 | <0.0001 | 0.0002 |
Data are mean ± SD (n = 3). H-1 = hypoxia for 1 h; H-2 = hypoxia for 2 h; H-3 = hypoxia for 3 h (at 37°C).