TABLE 1

Data for 2-Step Radioiodination with 2.04–4.81 GBq (55–130 mCi) of ¹³¹I

GBq of ¹³¹I	GBq recovered (% recovery)	Specific activity (MBq/mg)	%
GBq of ¹³¹I	GBq recovered (% recovery)	Specific activity (MBq/mg)			¹³¹I-IMP-R4-hMN-14*	Immunoreactivity^†
2.04	1.20 (58.8)	188.7	97.8	98.6
2.08	1.39 (66.8)	204.6	96.5	100
2.09	1.44 (68.9)	208.7	97.7	99.0
2.10	1.32 (62.9)	212.8	96.8	ND
2.11	1.47 (69.7)	197.2	98.0	99.0
2.12	1.48 (69.8)	214.2	97.9	95.8
2.29	1.45 (63.3)	168.4	97.8	ND
2.31	1.00 (43.3)	138.4	99.0	99.0
2.42	1.43 (59.0)	178.7	98.8	99.5
2.42	1.29 (53.3)	230.5	94.9	ND
2.60	1.49 (57.3)	184.3	98.0	99.0
3.73	2.23 (59.8)	182.8	98.0	97.7
4.03	1.94 (48.6)	212.0	98.0	95.0
4.05	2.09 (51.7)	166.5	98.0	95.0
4.06	2.56 (63.1)	202.8	99.4	95.9
4.37	3.03 (69.3)	222.7	98.0	97.2
4.55	2.33 (51.2)	212.4	97.7	95.0
4.81	2.98 (61.9)	248.3	98.1	100

↵* Percentge incorporation into hMN-14 in purified product (determined by HPLC), with aggregate content of 0%–4%.
↵^† Determined by percentage HPLC peak shift after complexation with CEA.
ND = not determined.