Nucleoside | TK specificity (relative to thymidine) (%) |
---|---|
Thymidine | 100 |
FLT | 43 |
FMAU | 31 |
FBAU | 37 |
Nucleosides were incubated with whole-cell protein extracts from PC3 cells for 1 h at 37°C. Nucleotides were separated on a DE-81 anion filter paper and counted on a scintillation counter. Activity values were normalized by the protein concentrations and averaged over 3 experiments.