TABLE 2

Analytic Data for (Radio)metallated Peptide-Conjugates

PeptideFormulaMW MALDI-TOF (%, m/z)HPLC tR (min)
System 2*System 3†
[99mTc]Demogastrin 1[99mTc]2N40,(D)Glu1]MGnd16.727.1
[ReV]Demogastrin 1[ReO2N40,(D)Glu1]MG2,065.916.0nd
2,088.0 (100, [M+Na]+)
[99mTc]Demogastrin 2[99mTcO2N40–1,Gly0(D)Glu1]MGnd20.826.9
[ReV]Demogastrin 2[ReO2N40–1,Gly0(D)Glu1]MG2,123.020.2nd
2,208.0 (100, [M+2Na+K]+)
[99mTc]Demogastrin 3[99mTc]2N′40,(D)Glu1]MG‡nd21.327.6
[ReV]Demogastrin 3[ReO2N′40,(D)Glu1]MG2,243.0§21.6nd
2,242.0

  • ↵* System 2 = XTerra RP-8 (5 μm, 4.6 × 150 mm) analytic cartridge column eluted at 1 mL/min with: 0%–40% B in 40 min A (A = 0.1% TFA in H2O, B = acetonitrile).

  • ↵† System 3 = Symmetry Shield RP18 (5 μm, 3.9 × 150 mm) analytic cartridge column eluted at 1 mL/min with: 0%–40% B in 40 min (A = 0.2% H3PO4 neutralized to pH 7 with 25% aq. NH3).

  • ↵‡ N′4 = (H2NCH2CH2NHCH2)CH-(p-CH2C6H4)-NHCOCH2OCH2CO-.

  • ↵§ ES-MS spectra were conducted.

  • MW = molecular weight; MG = minigastrin; nd = not done.