Biodistribution of 99mTc-Labeled NAP-2 and 3 C-Terminally Truncated Variants in Rabbits with Escherichia coli—Induced Intramuscular Infection
| Parameter | 99mTc-NAP-2 | 99mTc-NAP-2(1–68) | 99mTc-NAP-2(1–66) | 99mTc-NAP-2(1–63) |
|---|---|---|---|---|
| Biodistribution | ||||
| Blood | 0.015 ± 0.001 | 0.013 ± 0.002 | 0.017 ± 0.003 | 0.013 ± 0.002 |
| Muscle | 0.0017 ± 0.0002 | 0.0016 ± 0.0002 | 0.0024 ± 0.0001 | 0.0018 ± 0.0003 |
| Abscess | 0.084 ± 0.015 | 0.098 ± 0.010 | 0.189 ± 0.044 | 0.114 ± 0.017 |
| Lung | 0.057 ± 0.005 | 0.081 ± 0.015 | 0.171 ± 0.026 | 0.074 ± 0.016 |
| Spleen | 0.15 ± 0.02 | 0.20 ± 0.05 | 0.30 ± 0.06 | 0.17 ± 0.03 |
| Kidney | 3.1 ± 0.1 | 3.0 ± 0.1 | 1.9 ± 0.1 | 2.3 ± 0.1 |
| Liver | 0.032 ± 0.001 | 0.035 ± 0.001 | 0.048 ± 0.006 | 0.043 ± 0.007 |
| Intestine | 0.011 ± 0.001 | 0.012 ± 0.001 | 0.016 ± 0.002 | 0.014 ± 0.001 |
| Ratio | ||||
| Abscess/muscle | 52 ± 11 | 65 ± 11 | 80 ± 23 | 66 ± 14 |
| Abscess/blood | 5.7 ± 1.3 | 8.0 ± 1.1 | 12 ± 3 | 8.8 ± 1.2 |
Rabbits were injected with 20 MBq 99mTc-labeled chemokine, killed at 6 h after injection, and dissected. Tissue samples were weighed and counted in a γ-counter. Values are expressed as %ID/g tissue ± 1 SEM or as ratios thereof.