Blood Clearance of Fusion Proteins and Biotin-DOTA
| scFv-SA | Biotinylated ligand | %ID/g | Half-life* (h) | ||||
|---|---|---|---|---|---|---|---|
| 30 min | 20 h | t1/2 α | t1/2 β | ||||
| 125I-5F11-scFv-SA | — | 34.9 | 5.5 | 0.2 ± 0.1 | 9.3 ± 0.7 | ||
| 125I-B9F9-scFv-SA | — | 47.6 | 12.2 | 0.7 ± 0.1 | 13.4 ± 1.0 | ||
| 125I-CC49-scFv-SA | — | 35.2 | 10.1 | 0.4 ± 0.1 | 14.0 ± 0.9 | ||
| 5F11-scFv-SA | |||||||
| 300 μg | 111In-Biotin-DOTA | 0.7 | 0.02 | 0.1 ± 0.01 | 4.6 ± 3.2 | ||
| 900 μg | 111In-Biotin-DOTA | 1 | 0.02 | 0.14 ± 0.01 | 3.8 ± 0.7 | ||
↵* Mean ± SEM.
Blood clearance studies were carried out in tumor-free nude mice. Half-lives were calculated for the respective radiolabeled ligand using Origin 6.0 software (Microcal Software).