Percentage Decrease in Self-Quenching with Time
| Time (d) | Zwitterionic liposomes Δq × 100* | Cationic liposomes Δq × 100* | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 800 nm | 400 nm | 100 nm | 800 nm | 400 nm | 100 nm | |||||
| 1 | 0 ± 2 | 0 ± 2 | 0 ± 3 | 0 ± 2 | 0 ± 2 | 0 ± 2 | ||||
| 2 | 11 ± 2 | 14 ± 3 | 13 ± 3 | 16 ± 3 | 20 ± 2 | 20 ± 3 | ||||
| 3 | 14 ± 2 | 14 ± 3 | 3 ± 3 | 14 ± 3 | 19 ± 3 | 17 ± 3 | ||||
| 10 | 18 ± 2 | 18 ± 2 | 19 ± 3 | 17 ± 5 | 25 ± 2 | 24 ± 3 | ||||
| 20 | 17 ± 2 | 16 ± 2 | 18 ± 3 | 20 ± 2 | 24 ± 2 | 26 ± 2 | ||||
| 30 | 14 ± 3 | 15 ± 3 | 19 ± 6 | 19 ± 4 | 22 ± 4 | 27 ± 6 | ||||
↵* Fractional fluorescence self-quenching decrease due to calcein leakage from pegylated liposomes over time (Δqday_x = (qday_1 − qday_x)/qday_1). The uncertainties correspond to SEs of repeated measurements.
legend After first 24 h, a maximum of 20% in self-quenching decrease was measured for all liposomes. Beyond this point, all liposomes were stable for more than 30 days.