Peptide-PNA-Peptide Chimera Characterization
| Name | Sequence | Label | Yield (%) | Mass (Da) | ||
|---|---|---|---|---|---|---|
| Calculated | Measured | |||||
| PNA-free | Glyd(Ala)GlyGlyAba-(Gly)4-d(CysSerLysCys) | WT990 | 19.0 | 990.0 | 992.0 | |
| PNA mismatch | AcGlyd(Ala)GlyGlyAba-CTGGACAACCAT-AEEA-d(CysSerLysCys) | WT4172 | 39.1 | 4,172.0 | 4,174.1 | |
| Peptide mismatch | AcGlyd(Ala)GlyGlyAba-CTGGTGTTCCAT-AEEA-d(CysAlaAlaCys) | WT4113 | 34.0 | 4,113.0 | 4,113.7 | |
| PNA antisense | AcGlyd(Ala)GlyGlyAba-CTGGTGTTCCAT-AEEA-d(CysSerLysCys) | WT4185 | 30.6 | 4,185.0 | 4,187.2 | |
| Fl-peptide mismatch | SFX-AEEA-CTGGTGTTCCAT-AEEA-d(CysAlaAlaCys) | WT4361 | 3.0 | 4,361.0 | 4,360.6 | |
| Fl-PNA antisense | SFX-AEEA-CTGGTGTTCCAT-AEEA-d(CysSerLysCys) | WT4433 | 2.8 | 4,433.0 | 4,433.8 | |
Fl = fluoresceinyl.