Comparison of Uptake in HSV-tk Transduced Tumor
| Tissue | Untreated (saline) | Treated (GCV) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 14C-ACPC K1 (μL/min/g) | 14C-ACPC ∂K1 (μL/min/g) | 67Ga-DTPA K1 (μL/min/g) | 18F-FDG R (μmol/hg/min) | 14C-ACPC K1 (μL/min/g) | 14C-ACPC ∂K1 (μL/min/g) | 67Ga-DTPA K1 (μL/min/g) | 18F-FDG R (μmol/hg/min) | |||||||
| Tumor | ||||||||||||||
| Whole tumor | 34.5 ± 9.4 (5)*† | 33.8 ± 8.9 (5)*† | 0.182 ± 0.099 (5)† | 25.4 ± 7.3 (5) | 9.8 ± 2.7 (6)‡ | 9.5 ± 2.7 (6)‡ | 0.115 ± 0.051 (6) | 12.3 ± 3.8 (5)*†‡ | ||||||
| Viable tumor | 35.2 ± 9.6 (5)*† | 34.9 ± 9.7 (5)*† | 0.168 ± 0.014 (5)*† | 26.1 ± 7.5 (5) | 9.7 ± 2.8 (6)‡ | 9.5 ± 2.8 (6)‡ | 0.096 ± 0.019 (6)†‡ | 13.1 ± 3.8 (5)*†‡ | ||||||
| Impending necrosis | 50.4 ± 18.9 (4) | 44.5 ± 20.1 (4) | 0.87 ± 0.19 (4) | 37.3 ± 10.6 (4) | 13.7 ± 7.4 (4) | 11.6 ± 5.1 (4) | 1.12 ± 1.43 (4) | 18.1 ± 9.0 (4) | ||||||
| Necrotic tumor | — | — | — | — | 1.0 ± 1.3 (5)§ | 1.3 ± 1.2 (5)§ | −0.16 ± 0.61 (5) | 4.5 ± 1.5 (5)*†‡§ | ||||||
| Contralateral brain | ||||||||||||||
| Frontal cortex | 18.9 ± 4.2 (5) | 18.8 ± 4.2 (5) | 0.081 ± 0.019 (5) | 42.1 ± 15.1 (5) | 10.9 ± 3.0 (6)‡ | 10.7 ± 3.0 (6)‡ | 0.092 ± 0.004 (6) | 30.0 ± 12.4 (5) | ||||||
| Corpus callosum | 14.9 ± 4.1 (5) | 14.8 ± 4.1 (5) | 0.054 ± 0.011 (5) | 22.9 ± 7.5 (5) | 8.2 ± 3.2 (6)‡ | 8.2 ± 3.2 (6)‡ | 0.059 ± 0.018 (6) | 18.9 ± 7.7 (5) | ||||||
↵* Statistically significant value compared with corresponding contralateral frontal cortex (P < 0.05, paired t test).
↵† Statistically significant value compared with corresponding contralateral corpus callosum (P < 0.05, paired t test).
↵‡ Statistically significant value compared with untreated rats (P < 0.05).
↵§ Significantly different from whole-tumor and viable tumor values (P < 0.05, paired t test).
Initial transport (clearance) constant, K1, was calculated from 10-min 14C-ACPC and 10-min 67Ga-DTPA experiments (Eq. 1); ∂K1 of ACPC was calculated (Eq. 2), and relative glucose utilization (R) was calculated from 60-min 18F-FDG data (Eq. 3; hg = 100 g). Data are shown for untreated (saline) control animals and for animals treated with GCV. Values are the mean ± SD; number of animals is given in parenthesis. One saline-treated animal died during study, and 1 GCV-treated animal did not receive 18F-FDG because radiopharmaceutical was not available.