Comparison of Uptake of 3H-FLT and 14C-DG in Growth-Arrested Cells and in Cells Subcultured in Fresh Medium for 6 or 20 Hours
| Cell type | Growth state | % S phase | 3H-FLT (37.0 kBq/mL) | 14C-DG (18.5 kBq/mL) | ||||
|---|---|---|---|---|---|---|---|---|
| dpm/106 cells | Uptake ratio (relative to plateau cells) | dpm/106 cells | Uptake ratio (relative to plateau cells) | |||||
| A549 | 8-d-old, unfed, plateau | 4 | 1,640 ± 39 | 1 | 5,244 ± 16 | 1 | ||
| Lung carcinoma | 6-h in fresh medium | 4 | 1,808 ± 81 | 1.1 | 6,688 ± 23 | 1.3 | ||
| 20-h in fresh medium | 32 | 10,430 ± 264 | 6.4 | 9,366 ± 166 | 1.8 | |||