Detailed Results of Model Parameters Estimated from the 8 Multiinjection Experiments
| Experiment | B′max (pmol/mL) | k1 (min−1) | k2 (min−1) | kon/VR (mL/[pmol min]) | koff (min−1) | FV | KdVR (pmol/mL) |
|---|---|---|---|---|---|---|---|
| 1 | 17.5 | 0.32 | 6.1 | 0.74 | 0.020 | 0.47 | 0.027 |
| 2 | 11.6 | 0.72 | 4.0 | 0.85 | 0.020 | 0.57 | 0.023 |
| 3 | 11.5 | 0.80 | 1.7 | 0.68 | 0.022 | 0.20 | 0.036 |
| 4 | 8.8 | 0.57 | 1.2 | 0.51 | 0.018 | 0.25 | 0.035 |
| 5 | 17.4 | 1.32 | 2.2 | 0.33 | 0.015 | 0.78 | 0.046 |
| 6 | 12.1 | 0.81 | 1.3 | 0.30 | 0.019 | 0.39 | 0.063 |
| 7 | 10.0 | 0.58 | 2.1 | 0.76 | 0.018 | 0.52 | 0.024 |
| 8 | 12.9 | 0.96 | 3.1 | 0.58 | 0.016 | 0.20 | 0.028 |
| Mean | 12.7 | 0.76 | 2.7 | 0.59 | 0.018 | 0.42 | 0.035 |
| SD | 3.2 | 0.30 | 1.7 | 0.20 | 0.002 | 0.20 | 0.013 |
Table 1 shows doses used. Model parameters are defined in Figure 1 legend.